A method for selective stimulation of leg chemoreceptors in whole crustaceans

Solari, Paolo
Primo
;
Sollai, Giorgia;Palmas, Francesco;Sabatini, Andrea;Crnjar, Roberto
Ultimo
2021

Abstract

The integration of sensory information with adequate motor outputs is critical for animal survival. Here, we present an innovative technique based on a non-invasive closed-circuit device consisting of a perfusion/stimulation chamber chronically applied on a single leg of the crayfish Procambarus clarkii. Using this technique, we focally stimulated the leg inside the chamber and studied the leg-dependent sensory-motor integration involving other sensory appendages, such as antennules and maxillipeds, which remain unstimulated outside the chamber. Results show that the stimulation of a single leg with chemicals, such as disaccharides, is sufficient to trigger a complex search behaviour involving locomotion coupled with the reflex activation of antennules and maxillipeds. This technique can be easily adapted to other decapods and/or other sensory appendages. Thus, it has opened possibilities for studying sensory-motor integration evoked by leg stimulation in whole aquatic animals under natural conditions to supplement, with a direct approach, current ablation/silencing techniques.
Inglese
224
J Exp Biol jeb.243636.
Esperti anonimi
internazionale
scientifica
Aquatic chemoreception; Leg response; Antennules; Maxillipeds; Behaviour
no
Solari, Paolo; Sollai, Giorgia; Palmas, Francesco; Sabatini, Andrea; Crnjar, Roberto
1.1 Articolo in rivista
info:eu-repo/semantics/article
1 Contributo su Rivista::1.1 Articolo in rivista
262
5
none
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