Titolo:  In Vitro Anti-HIV-1 Reverse Transcriptase and Integrase Properties of Punica granatum L. Leaves, Bark, and Peel Extracts and Their Main Compounds.
Data di pubblicazione:  2021
Data di prima pubblicazione on-line:  2021
Autori:  Sanna, C.; Marengo, A.; Acquadro, S.; Caredda, A.; Lai, R.; Corona, A.; Tramontano, E.; Rubiolo, P.; Esposito, F.
Numero degli autori:  9
Lingua:  Inglese
Presenza coautori internazionali:  no
Rivista:  PLANTS
Volume:  10
Numero dell’articolo:  2124
Pagina iniziale:  1
Pagina finale:  11
Numero di pagine:  11
Digital Object Identifier (DOI):  http://dx.doi.org/10.3390/plants10102124
Codice identificativo Scopus:  2-s2.0-85103290368
Codice identificativo ISI:  WOS:000711395700001
Abstract:  In a search for natural compounds with anti-HIV-1 activity, we studied the effect of the ethanolic extract obtained from leaves, bark, and peels of Punica granatum L. for the inhibition of the HIV-1 reverse transcriptase (RT)-associated ribonuclease H (RNase H) and integrase (IN) LEDGFdependent activities. The chemical analyses led to the detection of compounds belonging mainly to the phenolic and flavonoid chemical classes. Ellagic acid, flavones, and triterpenoid molecules were identified in leaves. The bark and peels were characterized by the presence of hydrolyzable tannins, such as punicalins and punicalagins, together with ellagic acid. Among the isolated compounds, the hydrolyzable tannins and ellagic acid showed a very high inhibition (IC50 values ranging from 0.12 to 1.4 microM and 0.065 to 0.09 microM of the RNase H and IN activities, respectively). Of the flavonoids, luteolin and apigenin were found to be able to inhibit RNase H and IN functions (IC50 values in the 3.7–22 microM range), whereas luteolin 7-O-glucoside showed selective activity for HIV-1 IN. In contrast, betulinic acid, ursolic acid, and oleanolic acid were selective for the HIV-1 RNase H activity. Our results strongly support the potential of non-edible P. granatum organs as a valuable source of anti-HIV-1 compounds.
Parole chiave:  Punica granatum; pomegranate; leaves; bark; peel; HIV-1 reverse transcriptase; HIV-1; integrase; ellagic acid; hydrolyzable tannins; triterpenoids
Tipologia: 1.1 Articolo in rivista

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