8167 - BIOCHEMISTRY AND MOLECULAR BIOLOGY
Academic Year 2019/2020
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ANDREA RINALDI (Tit.)
- Teaching style
- Lingua Insegnamento
|[40/39] MEDICINE AND SURGERY||[39/00 - Ord. 2015] PERCORSO COMUNE||12||120|
Knowledge of the biomolecules as the organic compounds from which living organism are constructed and that derived from biological activities. Knowledge of structure, properties, function, interactions and metabolism of biomolecules.
Knowledge of genes and genomes structure and function. Key topic on mammalian gene expression
Knowledge of DNA technologies in Medical Sciences (Medicine)
The students must know the basic principles of Physics, Chemistry, Biology and Genetics
Introduction to biochemistry (biomolecules; water effect on dissolved biomolecules; weak interactions in aqueous systems).
Structure and function of carbohydrates (monosaccharides, disaccharides, polysaccharides; proteoglycans, glycoproteins and glycolipids).
Structure and function of lipid (fatty acids, storage lipids, structural lipids in membranes, cholesterol; lipids with specific biological activity; fat-soluble vitamins; lipoproteins).
Structure and function of proteins (aminoacids and peptide bond; peptides and proteins; primary, secondary, tertiary and quaternary structure; alfa-keratin, collagen and elastin; myoglobin and haemoglobin; oxygen and carbon dioxide transport; immunoglobulins).
Structure and function of water-soluble vitamins and their coenzymatic derivatives.
Enzymes (apoenzyme, cofactors and coenzymes, holoenzyme; enzyme-substrate complex and active site; enzymatic reaction mechanisms; enzyme kinetics; enzyme classification; several factors that affect the rate of enzyme-catalyzed reactions; enzyme activity regulation).
Introduction to metabolism (endergonic and exergonic reactions; catabolic and anabolic pathways; energy production and consumption in metabolism; role of ATP and other phosphorilated compounds; metabolism integration and regulation).
Carbohydrate metabolism (glycolysis and gluconeogenesis; alcohol and lactic fermentations and pyruvate oxidation to acethyl-CoA; the pentose phosphate pathway; glycogen lysis and glycogen synthesis).
Lipid metabolism (digestion, mobilization and transport; fatty acids: activation and transport into mitochondria; β-oxidation of saturated/unsaturated fatty acids, with even/odd number of carbon atoms; propyonyl-CoA fate; ketones bodies: formation and utilization; biosynthesis of cholesterol and fatty acids).
Aminoacid metabolism (transamination and oxidative deamination; amino acid carbon skeletons oxidation: glucogenic and ketogenic amino acids; metabolic fates of amino groups;; urea cycle; amino acid converted to biological amines by decarboxilation).
Energy production (citric acid cycle, oxidative phosphorilation: mitochondrial respiratory chain, mitochondrial electron flow coupled to proton gradient; ATP synthase complex; shuttle systems for mitochondrial oxidation of cytosolic NADH).
Nucleic acids: Structure and function
DNA hybridization, molecular probes
Restrictions enzymes, Southern blotting
Cloning vectors, gene , genomic libraries
Polymerase Chain Reaction (PCR)
Molecular methods for mutations detection
DNA sequencing by Sanger’s Method and Next Generation Sequencing (NGS) Methods
Human Genome sequencing and its implications for human health
Genomics, Transcriptomics, Proteomics, Farmacogenomics
Transcriptional regulation and gene expression in eukaryotes:
Trascriptional initiation complex: assembling and regulation
Controlling chromatin structure: remodeling complexes and chromatin modifiers
DNA methyation and genomic imprinting, imprinting diseases and molecular diagnosis
PremRNA maturation and gene expression regulation:
One gene many proteins: alternative splicing, promoter selection, RNA editing, start translation selection.
Post-transcriptional regulation of gene expression:
Cytoplasmic regulation of mRNA degradation
The immune system: somatic DNA recombination and genes expression.
Frontal lectures/interactive learning/ laboratory experience
Verification of learning
Oral discussion; Final oral examination about all the topics included in the course; Score in 30th
Nelson-Cox; I Principi di Biochimica di Lehninger; Zanichelli
Voet-Voet-Pratt; Fondamenti di Biochimica; Zanichelli
Garrett-Grisham; Principi di Biochimica; Piccin
Mathews-Van Holden- Ahern; Biochimica; Casa Editrice Ambrosiana
Berg-Tymoczko-Stryer; Biochimica; Zanichelli
Allison LA, Fondamenti di Biologia molecolare (Zanichelli)
Lewin B, Il Gene (ed compatta) (Zanichelli)
Watson JD Biologia molecolare del gene (Zanichelli)
BIOCHIMICA CORSO PARI
Prof.ssa R. Medda (email@example.com) Dipartimento di Scienze della Vita e dell’Ambiente, Macrosezione Biomedica Cittadella universitaria di Monserrato
BIOLOGIA MOLECOLARE CORSO PARI
Prof.ssa A. Coiana (firstname.lastname@example.org ) Dipartimento di Sanità pubblica, Medicina clinica e molecolare, Sezione di Scienze Biomediche e Biotecnologie, Via Jenner s/n Cagliari
BIOCHIMICA CORSO DISPARI
Prof. A. Rinaldi (email@example.com ) Dipartimento di Scienze biomediche, Sezione di Biochimica, Cittadella universitaria di Monserrato
BIOLOGIA MOLECOLARE CORSO DISPARI
Prof. M.C. Rosatelli (firstname.lastname@example.org) Dipartimento di Sanità pubblica, Medicina clinica e molecolare, Sezione di Scienze Biomediche e Biotecnologie, Via Jenner s/n Cagliari
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