LITHIUM AND THIOCOLCHICOSIDE INTERACTION ON RAT DENTATE GYRUS NEURONAL NETWORK EXCITABILITY

FRAU, ALDO;Sanna E.
2010

Abstract

The muscle relaxant thiocolchicoside (TCC) is commonly used in clinical practice also for its anti-inflammatory and analgesic effects. Several previous studies have proposed that TCC as a competitive antagonist of GABAARs, and in vivo exerts a proconvulsant and convulsant activity in rats and humans. Preliminary data suggest that pre treatment of rats with concentration of lithium similar to those found in humans under clinical conditions, markedly enhances the convulsant effects of TCC. To better understand the nature of the interaction between lithium and TCC, we performed extracellular electrophysiological recordings of fEPSPs and evaluated the neuronal excitability in rat dentate gyrus (DG) slices. After 6 h of LiCl (1 mM) incubation the I/O responses of fEPSPs were markedly enhanced compared to control. Application of 1 M TCC potentiated fEPSPs with a greater efficacy in slices after LiCl incubation. Patch-clamp recordings in DG granule cells revealed that LiCl incubation enhanced the frequency of mEPSCs and reduced paired-pulse facilitation ratio suggesting an increased probability of glutamate release. Taken together these results indicate that treatment of slices with LiCl increases fEPSP recorded from DG granule cells, and markedly potentiates the effects of TCC. The strong increase of DG neuronal excitability induced by the association of lithium and TCC is consistent with the pharmacological observation of an enhanced convulsant activity in rats and suggests that particular attention should be taken when such drug combination is used in clinical practice.
Inglese
Volume 19 June 2010
International Behavioral Neuroscience Society, Volume 19 June 2010
June 8-13, 2010
Villasimius, Sardinia, Italy
internazionale
no
274
Obili, N; Fadda, E.; Uras, R.; Cordeddu, G.; Fois, C.; Prinzis, S.; Frau, Aldo; Piras, V.; Siuni, L.; Niola, P.; De Riu, Pl; Sechi, Gp; Sanna, E.
4.2 Abstract in Atti di convegno
4 Contributo in Atti di Convegno (Proceeding)::4.2 Abstract in Atti di convegno
13
info:eu-repo/semantics/conferenceObject
none
Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Questionnaire and social

Share on:
Impostazioni cookie